Thorlabs Multiphoton

Microscope frame: Thorlabs Bergamo upright

Objectives: 20x/1.0 W, adpter is available for other standard objectives

2-Photon laser: Coherent Chameleon Vision II

2-stagelaser intensity adjustment:  waveplate (coarse) & Pockels cell (fine)

Laser wavelength range: 680 nm - 1080 nm

Imaging software: Thorlabs ThorimageLS

Signal detection modes: 2-channel backward signal , 2-channel forward signal, or 2-channel combined signal

Detectors: Thorlabs GaAsP (4 x PMT2101), two for backward signal and two for forward signal

Channel selection: adjustable by emission filter exchange

Bright-field: Thorlabs white LED

Fluorescence Light:  CoolLed pE-300 (DAPI, GFP and Alexa568/Cy5)

XY Stage: Thorlabs motorized stage

Axial scan: piezo objective scanner and step-motor microscope scanner

Live experiment support: N/A

​Optical bench: Thorlabs Active

Computer: CPU: Intel Xeon E5-2620, OS: Windows 10, RAM: 32 GB, local data storage: 1 TB

Sample holder: slide and 35mm petri-dish