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3i Spinning Disc

  • Microscope Frame: Olympus IX81

  • Objectives: 20x/0.5, 40x/0.95, 60x/1.35 Oil, 100x/1.4 Oil, 100x/1.49 Oil TIRF

  • Bright-field Mode: BF, DIC

  • Acquisition Software: Slidebook 6 (64-bit)

  • Auto-focusing: N/A

  • Live Experiment Support: Heated chamber (37 deg.) and 5% CO2 (humidified)

  • Optical Bench: Passive

  • Computer: Intel Xeon E5-2609(CPU), Windows 10(OS), 16GB(RAM), 1TB(local data storage)

  • Sample Holder: universal slide/dish, 96-well plate

  • Commercial Service Contract: N/A

Wide-field Imaging

 

  • Camera: ORCA ER (1344X1024 pixel, 12-bit, 8.3 fr/s)

  • Fluorescence Light Source: X-Cite TURBO with Laser, LED Hybrid Drive

  • Excitation Filter on IX81 Turret

    • DAPI - 377/50 (Pos 1)

    • eGFP - 485/20 (Pos 2)

    • Alexa 561 - 560/25 (Pos 3)

    • Cy5 - 650/13 (Pos 7)

    • BF - (Pos 6)

Spinning Disc Confocal

 

  • Camera: Evolve EMCCD (512X512 pixels, 16 bits, 33.7 fr/s)

  • Lasers: 405 nm, 488 nm, 561 nm, & 640 nm

  • Emission Filters

    • DAPI - 457 nm (Pos 3)​

    • eGFP - 528nm (Pos 4)

    • Alexa 561 - 617nm (Pos 5)

    • Cy5 - 700nm (Pos 6)

    • BF - (Pos 1)

    • DIC - Analyzer (Pos 7)

    • Quadband: 457/528/617/700 (Pos 2)
       

TIRF Imaging

 

  • Camera: ORCA ER (1344X1024 pixel, 12-bit, 8.3 fr/s)

  • Objective for TIRF: 60x/1.35 Oil, 100x/1.4 Oil, 100x/1.49 Oil TIRF

  • Lasers: 405 nm, 488 nm, 561 nm, & 640 nm

  • Dichroic (quadband dichroic/emission) filter cube: 457/528/617/700 (Pos 6)

Lab: JMS 2C 33/34

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