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Zeiss Apotome

  • Microscope frame: Zeiss Observer Z1

  • Objectives: 5x/0.15, 10x/0.25, 40x/1.3 Oil DIC, 63x/1.4 Oil, 100x/1.4 Oil DIC

  • Brightfield imaging: BF, DIC, and Phase Contrast (Ph1 only)

  • Camera: 2 X Flash 4.0 Lite (2048X2048 pixel, 16-bit, 30 fr/s)

  • Image splitter: TwinCam dual camera splitter with 565nm beamsplitter (Cairn Research)

  • Fluorescence light source: Zeiss Colibri 7 LED

  • Acquisition software: Zeiss Zen (blue)

  • Image Processing Software: Zeiss Zen Lite (blue)

  • Auto-focusing: Zeiss Definite Focus 2 (hardware)

  • Background rejection: Zeiss Apotome Inset

  • Live experiment support: heated live chamber (37 deg.) and 5% CO2 (humidified)

  • Optical bench: API Passive

  • Computer: CPU: Intel Xeon E5-2643, OS: Windows 10, RAM: 16 GB, local data storage: 1.81 TB (RAID 0)

  • Sample holder: universal slide/dish, 96-well plate

  • Maintenance contract: Zeiss Protect Premium (microscope, stage and computer) Until 28/02/2027

Apotome-15th.jpg

Filter cube settings

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  • DAPI - G365-FT395-BP445/50 (Pos 1)

  • eGFP - BP470/40-FT495-BP525/50 (Pos 2)

  • Alexa 561 -  BP550/25-FT570-BP605/70 (Pos 3)

  • Cy5 - BP640/30-FT660-BP690/50 (Pos 4)

  • BF - N/A

Condenser settings

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  • PH1, PH2 (Pos 1)

  • BF (Pos 2)

  • IX DIC-20 (Pos3)

  • IX DIC-40 (Pos 3)

  • IX DIC-60 (Pos 4)

Lab: GDSC G3.07  

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