Dragonfly Spinning Disc
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Microscope frame: Leica DM6 FS
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Spinning disc unit: Andor Dragonfly 200
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Random access active illumination unit: Andor Mosaic 3
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Objectives: 10x/0.32, 25x/0.95 W, 40x/0.8 W U-V-I, 63x/0.9 W U-V-I, 63x/1.4-0.60 Oil
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Bright-field Mode: BF and ICT
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Camera:
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iXon 888 Life EMCCD (1024X1024 pixel, 16-bit, 26 fr/s),
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Zyla 4.2 plus sCOMS (2048X2048 pixel, 16-bit, 53 fr/s)
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Fluorescence light source: Coolled pe-300 white
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Acquisition software: Fusion
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Image processing software: Imaris (inbeded in Fusion)
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Image deconvolution software: Autoquant (inbeded in Fusion)
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Super-resolution Imaging: Real time SRRF
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Auto-focusing: N/A
Spinning disc confocal unit
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Switchable 40 um, 25 um pinhole disc
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Parkable spinning disc for widefield
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Switchable dual camera
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Separate filter wheel for each camera
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565 nm long-pass & 565 short-pass dichroics
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Lasers: 100 mW 405nm diode, 50mW 488nm diode, 50mW 561nm OPSS and 140mW 637 diode
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Emission Filters​
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DAPI - 2 x BP445/50 (Pos 1)
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eGFP - 2 x BP525/50 (Pos 2)
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Alexa 561 - 2 x BP605/70 (Pos 3)
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Cy5 - 2 x BP690/50 (Pos 4)
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Mosaic 3 unit
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256 x 256 pixels MMC mirror array for photo activation and photobleaching
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Mannually switchable light source
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Activation lasers:
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1.1W 405nm
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Port for 2nd laser​​​​
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Dichroic on microscope turret:​ 405/488/561/640
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Microscope condenser:
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Ph1 (Pos 1)
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BF (Pos 2)
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IX-DIC20 (Pos 3)
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IX-DiC40 (Pos 4)
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IX-DIC60 (Pos 5)
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Focusing fluoresence light: Coolled pe-300 white
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Live experiment support: Heated live chamber (37 deg.), Faraday magnetic shield, and 5% CO2 (humidified)
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Optical bench: active
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Computer
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CPU: 2 x Intel Xeon E5-2637 @3.5GHz
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OS: Windows 10, RAM: 96 GB
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Local storage: 1.81 TB (RAID 0)
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GPU: NVUDIA Quadro M4000, AMD Radeon ProWx 7100
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Sample holder: slide, & 35 mm Petr dish
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Commercial service contract: N/A
Lab: CRPC 3.16