Nikon E400

Microscope Frame: Nikon E400

Objectives: 4x/0.1, 10x/0.25, 40x/0.65, 100x/1.3 Oil

Fluorescence Excitation: pE-300 LED

Bright-field Setup: BF

Camera: ORCA-05G (Hamatsu CCD: 1344x1024 pixels, 12 bits, 8.9 fr/s)

Fluorescence channels: DAPI, eGFP, Alexa 561, & Cy5

Acquisition Software: Micro-Manager

Auto-focusing: N/A

Live Experiment Support: N/A

Optical Bench: N/A

Computer: CPU: Intel Core i5-4570, OS: Windows 10, RAM: 8 GB, local data storage: 1 TB

Sample Holder: universal slide/dish