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Olympus IX71

  • Microscope Frame: Olympus IX71

  • Objectives: 10x/0.3, 20x/0.45, 40x/?, 60x/1.4 Oil*, 100x/1.4 Oil* 

  • Fluorescence Excitation: CoolLed pE-4000

  • Bright-field Imaging: BF, DIC

  • Camera: Prime ( Photometrics sCOMS: 2048x2048 pixels, 16 bits, 30 fr/s)

  • Acquisition Software: Micro-Manager

  • Auto-focusing: Software based

  • Live Experiment Support: Heated live chamber (37 deg.) and 5% CO2 (humidified)

  • Optical Bench: Passive

  • Computer: Dell, CPU: Intel i7-6700, OS: Windows 10, RAM: 32 GB, local data storage: 2 TB (RAID 0)

  • Sample Holder: universal slide/dish, 96-well plate

  • Commercial Service Contract:  N/A

IX71_edited.jpg

Excitation filters

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  • DAPI - 395/25 nm

  • eCFP - 434/17 nm

  • eGFP - 470/20 nm

  • eYFP - 510/10 nm

  • Alexa 561 - 550/15 nm

  • Cy5 - 640/30 nm

  • BF & DiC - White LED 

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Eyepiece filters

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DAPI/eGFP/TRITC/Cy5 - 432/515/595/730 (Pos 2 Green, default)

eCFP - 457/50(Pos 1, blue)

Alexa 561 - 607/36 (Pos 3 Red)

eGFP-MCherry - ?(Pos 4)

DIC - Analyzer(Pos 5)

Emssion filters

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  • DAPI - 435/40 nm (Pos 7)

  • eCFP - 480/17 nm (Pos 3)

  • eGFP - 525/50 nm (Pos 8)

  • eYFP - 537/26 nm (Pos 4)

  • Alexa 561 - 617/73 nm (Pos 10)

  • Cy5 - 680/40 nm (Pos 5)

  • Sir 700 - 740/60 nm (Pos 6)

  • BF - Empty (Pos 2)

  • DiC -Analyzer (Pos 1)

Dichroic

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  • DAPI-eGFP-TRITC-Cy5 - 410/515/582/669 (Pos 2)

  • eCFP-eGFP-TRITC-Cy5 - 444/520/590 (Pos 1)

  • eGFP-HcRed - 520/633 (Pos 3)

  • DAPI-eGFP-TRITC-Cy5 - 409/493/573/652 (Pos 8 default)

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Condensor

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PH1: Pos 1

BF: Pos 2 (default)

IX2 DIC20: Pos3

IX2 DIC40: Pos 4

IX2: DIC60: Pos 5

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Lab: JMS 2C33/34

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