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Olympus IX73

  • Microscope Frame: Olympus IX73

  • Objectives: 10x/0.4, 20x/0.7, 40x/0.6, 40x/0.95

  • Fluorescence Excitation: CoolLed pE-4000

  • Bright-field Imaging: BF, PH(20x), DIC

  • Camera:  Flash4.0 Lite (2048x2048 pixels, 16 bits, 30 fr/s)

  • Acquisition Software: Micro-Manager

  • Auto-focusing: Software based

  • Live Experiment Support: Heated live chamber (37 deg.) and 5% CO2 (humidified)

  • Optical Bench: API Passive

  • Computer: Dell Precision 3620, CPU : Intel Core i7-6700, OS: Windows 10, RAM: 32 GB, local data storage: 2 TB (RAID 0)

  • Sample Holder: universal slide/dish, 96-well plate

  • Commercial Service Contract: N/A

IX73-15th.jpg

Excitation filter settings

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  • DAPI - Empty

  • eCFP - 440/20 nm

  • eGFP - 470/24 nm

  • eYFP - N/A

  • Alexa 561 - 550/15 nm

  • Cy5 - 640/40 nm

  • Sir 700 - 640/40 nm

  • BF - Empty

  • DIC - White LED

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pE-4000 wavelengths: 365 nm, 470 nm,

                                            550 nm, 635 nm

​​

Emission filter settings

 

  • DAPI - 435/40 nm (Pos 8)

  • eCFP - 480/17 nm (Pos 3)

  • eGFP - 514/30 nm (Pos 6)

  • eYFP - 537/26 nm (Pos 4)

  • Alexa 561 - 595/31 nm (Pos 7)

  • Cy5 - 685/40 nm (Pos 1)

  • BF - N/A (Pos 5)

  • DIC - Analyser (Pos 0)

  • SIR700 740/60 nm (Pos 9)

Dichroic cube

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  • DAPI/eGFP/TRITC/Cy5 - Di 439/507/576/668 (Pos 8 default)

  • eCFP/eYFP  - Di 440/520, Em FF464/547 (Pos 2)

  • eGFP/HcRed -Di 495/605, Em FF527/645 (Pos 3)

  • Alexa 561 - Di Q565LP, Em.584/14 (Pos 5)

  • TexasRed - Di AT610LP (Pos 6)

​​

 Eyepiece filters settings

  â€‹

  • DAPI/eGFP/TRITC/Cy5 - 444/520/595/703 (Pos 0)

  • eCFP/eYFP/eRFP - 469/535/637(Pos 1)

  • DIC - Analyzer (Pos 2)

  • BF - Empty (Pos 3)

Lab: JMS 2C 33/34

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