top of page

Zeiss LSM880

  • Microscope Frame: Zeiss Observer Z1

  • Objectives: 5x/0.15, 10x/0.45, 20x/0.8, 40x/0.6, 40x/1.3 Oil, 60x/1.4 Oil, 40x/1.2 W

  • Bright-field Imaging: BF, DIC

  • Auto-focusing: Zeiss Definite Focus 2 (hardware autofocus) and software autofocus

  • Axial Scan: Piezo stage, motorized objectives

  • Live Experiment Support: Heated live chamber (37 deg.) and 5% CO2 (humidified)

  • Optical Bench: Thorlabs Active

  • Computer: hp Z6, CPU: Intel Xeon Gold 6234, OS: Windows 10, RAM: 128 GB, local data storage: 8 TB

  • Control Software: Zeiss Zen (black)

  • Sample Holder: universal slide/dish, 96-well plate

  • Commercial Service Contract: Zeiss, Platinium until Feb 2027

Confocal Imaging

 

  • Lasers: 405 nm, 458 nm, 488 nm, 514 nm, 568 nm, 594 nm, & 633 nm

  • Detectors:

    • 32 GaAs Array

    • 2 x PMT

    • 1 x Trans PMT

  • Detection Mode: 

    • Confocal

    • Spectral Imaging

    • Spectral Unmixing​

​

Airyscan Imaging

 

  • Lasers: 405 nm, 458 nm, 488 nm, 514 nm, 568 nm, 594 nm, & 633 nm

  • Detectors: 32 GaAs Array

  • Acquisition: channel sequential

  • Detection mode

    • SR - Super resolution

    • R-S - S/N enhancement

    • VP - Virtual pinhole

    • CO - Array Confocal

​

​

Widefield Fluorescence Imaging

​

  • Camera: Axiocam 503 Mono (1936x1460 pixels, 14bits, 38 fr/s)

  • Control Software: Zeiss Zen (blue)

  • Light Source: Zeiss HXP 120V Mercury short-arc lamp unit

  • Filter Settings: 

    • DAPI - Fset 49 wf

    • eGFP - Fset 38 wf

    • Alexa 561 - Fset 43 wf

    • DIC - Analyser

Lab: JMS 2C31

bottom of page