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Revvity Operetta CLS

  • Microscope Frame: Operetta CLS

  • Objectives: 5x/0.16, 10x/0.3, 20x/0.4 LW, 20x/0.8, 40x/0.6 LW, 40x/1.1 W, 60x/1.15 W

  • Fluorescence Excitation: PerkinElmer LED (build-in)

  • Bright-field Mode: BF, Digital Phase Contrast

  • Camera: Andor Zyla 5.5 (2560x2160 pixels, 16 bits, 40 fr/s)

  • Acquisition Software: Harmony

  • Analysis Software: Harmony (2 offline copies)

  • Auto-focusing: Hardware based

  • Live Experiment Support: Heated live chamber (37 deg.) and 5% CO2 (humidified)

  • Optical Bench: N/A

  • Computer: CPU: Intel Xeon E5-2620, OS: Windows 10, RAM: 32 GB, local data storage (C:): 22 TB

  • Sample Holder:  4x slide holder (Maximum 4 slides), 96-well plate

  • Commercial Service Contract: Revvity Gold Service Plan, March 2023 - March 2026

  • Excitation: 365 nm, 440 nm, 475 nm, 510 nm, 550 nm, & 630 nm

  • Emission filters

    • DAPI - 430 - 500 nm

    • eCFP (FRET) - 473.5 - 514.5 nm

    • eGFP - 500-550 nm

    • eYFP - 517.5 - 572.5 nm

    • Alexa 561 - 570-650 nm

    • Cy5 - 655 - 760 nm​

  • BF light source: white LED

  • BF contrast enhancement: BF & digital phase

  • HCS 2D/3D quantification functions

  • Spreadsheet result output

  • Database shared with offline workstation for parallel data analysis​

Lab: JMS 2c 41   

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