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Olympus ScanR

  • Microscope Frame: Olympus IX83

  • Objectives: 10x/0.4 20x/0.45 Ph1, 40x/0.6 Ph2, 60x/0.7 Ph3, 60x/0.9

  • Fluorescence Excitation: Lumencor Spectra X

  • Bright-field Mode: BF,PH

  • Camera: ORCA-Flash4 LT3 (Hamamatsu sCMOS: 2048x2048 pixels, 16bits, 30 fr/s)

  • Acquisition Software: ScanR Acquisition (V 3.5.0)

  • Analysis Software: ScanR Analysis (V3.5.2, 2 copies)

  • Auto-focusing: Hardware (ZDC1) and software based auto-focusing

  • Live Experiment Support: Heated live chamber (37 deg.) and 5% CO2 (humidified)

  • Optical Bench: N/A

  • Computer: HP Z4, CPU: Intel Core i7-7800X, OS: Windows 10, RAM: 16 GB, local data storage: 7.27 TB

  • Sample Holder: universal slide/dish, 96-well plate

  • Commercial Service Contract: Olympus Maintenance Plan until 2029

  • Excitation filters

    • DAPI - 377/54 nm

    • eCFP - 438/29 nm

    • eGFP - 475/28 nm

    • eYFP - 510/25 nm

    • Alexa 561 - 555/28 nm

    • TxRed - 575/25 nm

    • Cy5 - 635/22 nm

    • Cy7 -740/20 nm

    • BF: white LED

  • Dichroic: FF410/504/582/669 (Pos 2)

  • Emission filters:

    • DAPI - 432/36 nm

    • eCFP - N/A

    • eGFP - 515/30 nm

    • eYFP - N/A

    • Alexa 561 - 595/33 nm

    • TxRed - N/A

    • Cy5 - 680/40 nm

    • Cy7 - N/A

  • Condensor

    • Ph1 (Pos 1)

    • PH2 (Pos 2)

    • BF (Pos 3)

  • Version of Software: V 3.5.0

  • ScanR Analysis: functions for image analysis​

  • AI based segmentation (neuron network)

  • Output of analysis result in spreadsheet

  • Offline workstation with 2nd copy of ScanR Analysis for parallel processing​

Lab: JMS 2C 41

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