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Olympus Scan R Robot

  • Microscope Frame: Olympus IX83

  • Objectives: 4x/0.16, 10x/0.4,  20x/0.5, 20x/0.8, 40x/0.75, 40x/0.6 

  • Fluorescence Excitation: Lumencor Spectra X

  • Bright-field Mode: BF, PH

  • Camera: ORCA-Fusion BT (Hamamatsu sCMOS: 2304x2304 pixels, 16bits, 89.1 fr/s)

  • Acquisition Software: ScanR Acquisition (V 3.5.0)

  • Analysis Software: ScanR Analysis (V3.5.2, 2 copies)

  • Auto-focusing: Hardware (ZDC1) and software based auto-focusing

  • Live Experiment Support: N/A

  • Optical Bench: Passive

  • Computer: HP Z32, CPU: Intel Core i9-10900X, OS: Windows 10 Pro, RAM: 32 GB, local data storage: 12 TB (RAID 0)

  • Sample Holder: universal slide/dish, 96-well plate

  • Maintenance Contract: Evident Maintenance Plan until 2029

ScanR_R.jpg

Excitation filters

  • DAPI - 390/22 nm

  • eCFP - 436/29 nm

  • eGFP - 475/28 nm

  • eYFP - 511/18 nm

  • Alexa 561 - 555/28 nm

  • TxRed - 575/25nm

  • Cy5 - 635/22 nm

  • BF: white LED​​

Emission filters

  • DAPI - 432/36 nm

  • eCFP - N/A

  • eGFP - 515/30 nme

  • YFP - N/A

  • Alexa 561 - 595/33 nm

  • TxRed - N/A

  • Cy5 - 698/70 nm

​

  • Dichroic: AHF F68-400 (435/518/594/694, Pos 1)

  • Robot Arm : ​2 x 8 wellplates or 2 x 8 x 4 slides

  • Version of Software: V 3.5.0

  • ScanR Analysis: functions for image analysis​

  • AI based segmentation (neuron network)

  • Output of analysis result in spreadsheet

  • Offline workstation with 2nd copy of ScanR Analysis for parallel processing​

Lab: JMS 2C41

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