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Abberior STED (expert line)

  • Microscope Frame: Olympus IX83

  • Objectives: UPLSAPO 20X/0.85 Oil, UAPON 40X340-2/1.35 Oil, UPLSAPO 100XO/1.4 Oil

  • Bright-field Imaging: BF, PO

  • Auto-focusing: Olympus IX3-ZDC-12/830 (hardware)

  • XY Stage: IX3-SSU Ultrasonic scanning stage

  • Axial Scan: Motorized objectives

  • Point Scanner: QUADScan Galvo scanner

  • Detector: 4 X APO

  • Imaging Software: Imspector LightBox

  • Live Experiment Support: N/A

  • Optical Bench: Thorlabs Active

  • Computer: CPU: Intel Xeon E3-1245, OS: Windows 10, RAM: 16 GB, local data storage: 1 TB

  • Sample Holder: universal slide holder (for 2 slides)

  • Maintenance Contract: N/A

STED_15th.jpg

Confocal Mode

 

  • Point scanning

  • Laser

    • 405 nm, cw/50mW

    • 488 nm, pulsed@40MHz/1mW

    • 561 nm, pulsed@40MHz/200uW

    • 640 nm, pulsed@40MHz/1mW

  • Emission filters

    • DAPI -  450/50e

    • GFP -  525/50

    • Alexa 568 -  605/50

    • Cy5 -  685/70

STED Mode

 

  • 2D and 3D STED

  • Adaptive illumination mode

    • RESCUE - Confocal

    • RESCUE - STED

    • DYMIN - 1 Step

    • DYMIN - 2 Step

  • Depletion Laser

    • 595 nm pulsed@40MHz/400mW

    • 775 pulsed@40MHz/2750mW

  • STED imaging channels

    • eGFP

    • Alexa 561

    • Cy5

Typical Fluorophores for STED 

 

  • Abberior Star Green

  • Abberior Star Orange

  • Abberior Star Red​

  • Alexa Fluor 594

  • Cy 3

  • Cy 5

  • Calibration Beads

    • TetraSpeck Spheres â€‹

    • Abberior Calibration Beads

Lab: JMS 2C33/34

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