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PALM/STORM

  • Microscope Frame: Olympus IX71

  • Objectives: 100x/1.49 Oil TIRF

  • Bright-field Mode: BF

  • Auto-focusing: MCL (hardware)

  • TIRF setup

  • Live Experiment Support: Heated live chamber (30 deg.) 

  • Optical Bench: Thorlabs Active

  • Sample Holder: universal slide/dish

  • Lasers: 405 nm, 488 nm, 561 nm, 633 nm

  • Camera: Kinetix 22 (2400 x 2400 pixels, 16 bit, BT, 664 fps)

  • 3D Prior stage

  • Prior 10-position emission filter wheel

  • Software: Micro-manager

PalmStorm_15th.jpg

  • Excitation Dichroics: 405 nm, 488 nm, 561 nm, & 630 nm (Pos 4)

  • Emission filters

    • DAPI - 488LP (Pos 0)

    • eGFP -525/45 (Pos 1)

    • Alexa 561 - 580/14 (Pos 2)

    • Cy5 - 647LP (Pos 7)

    • Cy5.5 692/40 (Pos 3)

    • TxRed -593/40 (Pos 4)

Lab: GDSC G2.10 

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